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On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli

dc.contributor.authorvan den Berg, Jonas
dc.contributor.authorGalbiati, Heloisa
dc.contributor.authorRasmussen, Akiko
dc.contributor.authorMiller, Samantha
dc.contributor.authorPoolman, Bert
dc.date.accessioned2016-09-06T15:00:07Z
dc.date.available2016-09-06T15:00:07Z
dc.date.issued2016-09-06
dc.identifier70218442
dc.identifier3f2fcc9e-929a-4401-93fb-46001c71c0d9
dc.identifier84986328544
dc.identifier.citationvan den Berg , J , Galbiati , H , Rasmussen , A , Miller , S & Poolman , B 2016 , ' On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli ' , Scientific Reports , vol. 6 , 32709 . https://doi.org/10.1038/srep32709en
dc.identifier.issn2045-2322
dc.identifier.otherORCID: /0000-0002-8223-9693/work/76974586
dc.identifier.urihttp://hdl.handle.net/2164/7449
dc.descriptionWe thank Frans Bianchi and Franz Ho for assistance with molecular cloning, Tim Rasmussen for providing the pTRC-MscK plasmid, Andrew Robinson for providing the pBAD-mEos3.2 plasmid, Matthias Heinemann for assistance with the flow cytometry measurements, Paul Schavemaker for performing Smoldyn simulations and Michiel Punter for programming ImageJ plugins for PALM reconstructions and single-particle tracking. We thank Ian Booth for critical reading of the manuscript, and Christoffer Åberg and Matteo Gabba for valuable discussions. The authors would like to thank David Dryden and Marcel Reuter for performing preliminary experiments from which this work has been built. The work was funded by the EU FP7 ITN-network program NICHE.en
dc.format.extent1158041
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.subjectmechanosensitive channelsen
dc.subjectosmoregulationen
dc.subjectsuper-resolution microscopyen
dc.subjectlateral diffusionen
dc.subjectquantitative biologyen
dc.subjectsingle-molecule analysisen
dc.subjectR Medicineen
dc.subject.lccRen
dc.titleOn the mobility, membrane location and functionality of mechanosensitive channels in Escherichia colien
dc.typeJournal articleen
dc.contributor.institutionUniversity of Aberdeen.Medical Sciencesen
dc.description.statusPeer revieweden
dc.identifier.doi10.1038/srep32709


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