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16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice

dc.contributor.authorWalker, Alan W.
dc.contributor.authorMartin, Jennifer C.
dc.contributor.authorScott, Paul
dc.contributor.authorParkhill, Julian
dc.contributor.authorFlint, Harry J.
dc.contributor.authorScott, Karen P.
dc.date.accessioned2015-06-29T11:30:00Z
dc.date.available2015-06-29T11:30:00Z
dc.date.issued2015-06-22
dc.identifier52593454
dc.identifiera0fd6506-f046-452a-b804-dbaec695d87f
dc.identifier84989245447
dc.identifier.citationWalker , A W , Martin , J C , Scott , P , Parkhill , J , Flint , H J & Scott , K P 2015 , ' 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice ' , Microbiome , vol. 3 , 26 . https://doi.org/10.1186/s40168-015-0087-4en
dc.identifier.otherORCID: /0000-0001-5099-8495/work/42316366
dc.identifier.urihttp://hdl.handle.net/2164/4656
dc.descriptionAcknowledgements The authors acknowledge the assistance of Grietje Holtrop (RINH-BioSS) with the statistical analysis of the data and the Wellcome Trust Sanger Institute’s 454 pyrosequencing team for generating 16S rRNA gene data. AWW, PS and JP received core funding support from the Wellcome Trust [grant number 098051]. AWW, JCM, HJF and KPS are funded by the Scottish Government (SG-RESAS).en
dc.format.extent1540322
dc.language.isoeng
dc.relation.ispartofMicrobiomeen
dc.subject16S rRNA gene sequencingen
dc.subjectbifidobacteriaen
dc.subjectinfanten
dc.subjectintestinal microbiotaen
dc.subjectFISHen
dc.subjectQH301 Biologyen
dc.subjectWellcome Trusten
dc.subject098051en
dc.subject.lccQH301en
dc.title16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choiceen
dc.typeJournal articleen
dc.contributor.institutionUniversity of Aberdeen.Rowett Instituteen
dc.description.statusPeer revieweden
dc.identifier.doi10.1186/s40168-015-0087-4


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