dc.contributor.author | Shahana, Shahida | |
dc.contributor.author | Childers, Delma S | |
dc.contributor.author | Ballou, Elizabeth R | |
dc.contributor.author | Bohovych, Iryna | |
dc.contributor.author | Odds, Frank C | |
dc.contributor.author | Gow, Neil A. R. | |
dc.contributor.author | Brown, Alistair J P | |
dc.date.accessioned | 2014-10-29T16:35:02Z | |
dc.date.available | 2014-10-29T16:35:02Z | |
dc.date.issued | 2014-06-18 | |
dc.identifier | 43200297 | |
dc.identifier | f706f501-0d54-463f-ba12-156d8e8f5fa8 | |
dc.identifier | 24940603 | |
dc.identifier | 84903289143 | |
dc.identifier.citation | Shahana , S , Childers , D S , Ballou , E R , Bohovych , I , Odds , F C , Gow , N A R & Brown , A J P 2014 , ' New Clox Systems for rapid and efficient gene disruption in Candida albicans ' , PloS ONE , vol. 9 , no. 6 , e100390 . https://doi.org/10.1371/journal.pone.0100390 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | ORCID: /0000-0002-2776-5850/work/52609456 | |
dc.identifier.other | ORCID: /0000-0003-2869-7332/work/76975981 | |
dc.identifier.uri | http://hdl.handle.net/2164/3786 | |
dc.description | Acknowledgements: We are grateful to Janet Quinn, Lila Kastora, Joanna Potrykus, Michelle Leach, and others for sharing their experiences with the Clox cassettes. We thank Julia Kohler for her kind gift of the NAT1-flipper plasmid pJK863, Claudia Jacob for her advice with In-fusion cloning, and our colleagues in the Aberdeen Fungal Group for numerous stimulating discussions. Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. The sequences of all Clox cassettes are available in GenBank: URA3-Clox (loxP-URA3-MET3p-cre-loxP): GenBank accession number KC999858. NAT1-Clox (loxP-NAT1-MET3p-cre-loxP): GenBank accession number KC999859. LAL (loxP-ARG4-loxP): GenBank accession number DQ015897. LHL (loxP-HIS1-loxP): GenBank accession number DQ015898. LUL (loxP-URA3-loxP): GenBank accession number DQ015899. Funding: This work was supported by the Wellcome Trust (www.wellcome.ac.uk): S.S., F.C.O., N.A.R.G., A.J.P.B. (080088); N.A.R.G., A.J.P.B. (097377). The authors also received support from the European Research Council [http://erc.europa.eu/]: DSC. ERB, AJPB (STRIFE Advanced Grant; C-2009-AdG-249793). The European Commission also provided funding [http://ec.europa.eu/research/fp7]: I.B., A.J.P.B. (FINSysB MC-ITN; PITN-GA-2008-214004). Also the UK Biotechnology and Biological Research Council provided support [www.bbsrc.ac.uk]: N.A.R.G., A.J.P.B. (Research Grant; BB/F00513X/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en |
dc.format.extent | 10 | |
dc.format.extent | 1652853 | |
dc.language.iso | eng | |
dc.relation.ispartof | PloS ONE | en |
dc.subject | QR Microbiology | en |
dc.subject | Wellcome Trust | en |
dc.subject | Biotechnology and Biological Sciences Research Council (BBSRC) | en |
dc.subject.lcc | QR | en |
dc.title | New Clox Systems for rapid and efficient gene disruption in Candida albicans | en |
dc.type | Journal article | en |
dc.contributor.institution | University of Aberdeen.Applied Medicine | en |
dc.contributor.institution | University of Aberdeen.Medical Sciences | en |
dc.description.status | Peer reviewed | en |
dc.identifier.doi | 10.1371/journal.pone.0100390 | |