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dc.contributor.authorShahana, Shahida
dc.contributor.authorChilders, Delma S
dc.contributor.authorBallou, Elizabeth R
dc.contributor.authorBohovych, Iryna
dc.contributor.authorOdds, Frank C
dc.contributor.authorGow, Neil A. R.
dc.contributor.authorBrown, Alistair J P
dc.date.accessioned2014-10-29T16:35:02Z
dc.date.available2014-10-29T16:35:02Z
dc.date.issued2014-06-18
dc.identifier.citationShahana , S , Childers , D S , Ballou , E R , Bohovych , I , Odds , F C , Gow , N A R & Brown , A J P 2014 , ' New Clox Systems for rapid and efficient gene disruption in Candida albicans ' , PloS ONE , vol. 9 , no. 6 , e100390 . https://doi.org/10.1371/journal.pone.0100390en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 43200297
dc.identifier.otherPURE UUID: f706f501-0d54-463f-ba12-156d8e8f5fa8
dc.identifier.otherPubMed: 24940603
dc.identifier.otherScopus: 84903289143
dc.identifier.otherORCID: /0000-0002-2776-5850/work/52609456
dc.identifier.otherORCID: /0000-0003-2869-7332/work/76975981
dc.identifier.urihttp://hdl.handle.net/2164/3786
dc.descriptionAcknowledgements: We are grateful to Janet Quinn, Lila Kastora, Joanna Potrykus, Michelle Leach, and others for sharing their experiences with the Clox cassettes. We thank Julia Kohler for her kind gift of the NAT1-flipper plasmid pJK863, Claudia Jacob for her advice with In-fusion cloning, and our colleagues in the Aberdeen Fungal Group for numerous stimulating discussions. Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. The sequences of all Clox cassettes are available in GenBank: URA3-Clox (loxP-URA3-MET3p-cre-loxP): GenBank accession number KC999858. NAT1-Clox (loxP-NAT1-MET3p-cre-loxP): GenBank accession number KC999859. LAL (loxP-ARG4-loxP): GenBank accession number DQ015897. LHL (loxP-HIS1-loxP): GenBank accession number DQ015898. LUL (loxP-URA3-loxP): GenBank accession number DQ015899. Funding: This work was supported by the Wellcome Trust (www.wellcome.ac.uk): S.S., F.C.O., N.A.R.G., A.J.P.B. (080088); N.A.R.G., A.J.P.B. (097377). The authors also received support from the European Research Council [http://erc.europa.eu/]: DSC. ERB, AJPB (STRIFE Advanced Grant; C-2009-AdG-249793). The European Commission also provided funding [http://ec.europa.eu/research/fp7]: I.B., A.J.P.B. (FINSysB MC-ITN; PITN-GA-2008-214004). Also the UK Biotechnology and Biological Research Council provided support [www.bbsrc.ac.uk]: N.A.R.G., A.J.P.B. (Research Grant; BB/F00513X/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofPloS ONEen
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/en
dc.subjectQR Microbiologyen
dc.subjectWellcome Trusten
dc.subjectBiotechnology and Biological Sciences Research Council (BBSRC)en
dc.subject.lccQRen
dc.titleNew Clox Systems for rapid and efficient gene disruption in Candida albicansen
dc.typeJournal articleen
dc.contributor.institutionUniversity of Aberdeen.Applied Medicineen
dc.contributor.institutionUniversity of Aberdeen.Medical Sciencesen
dc.contributor.institutionUniversity of Aberdeen.Institute of Medical Sciencesen
dc.contributor.institutionUniversity of Aberdeen.Clinical Medicineen
dc.description.statusPeer revieweden
dc.description.versionPublisher PDFen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0100390


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