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ACME dissociation : a versatile cell fixation-dissociation method for single-cell transcriptomics

dc.contributor.authorGarcía-Castro, Helena
dc.contributor.authorKenny, Nathan J.
dc.contributor.authorIglesias, Marta
dc.contributor.authorÁlvarez-Campos, Patricia
dc.contributor.authorMason, Vincent
dc.contributor.authorElek, Anamaria
dc.contributor.authorSchönauer, Anna
dc.contributor.authorSleight, Victoria A.
dc.contributor.authorNeiro, Jakke
dc.contributor.authorAboobaker, Aziz
dc.contributor.authorPermanyer, Jon
dc.contributor.authorIrimia, Manuel
dc.contributor.authorSebé-Pedrós, Arnau
dc.contributor.authorSolana, Jordi
dc.date.accessioned2022-12-01T00:05:42Z
dc.date.available2022-12-01T00:05:42Z
dc.date.issued2021-04-08
dc.identifier218812792
dc.identifier6deae597-06a0-4883-8eb4-8690848b03b2
dc.identifier85103998605
dc.identifier33827654
dc.identifier.citationGarcía-Castro , H , Kenny , N J , Iglesias , M , Álvarez-Campos , P , Mason , V , Elek , A , Schönauer , A , Sleight , V A , Neiro , J , Aboobaker , A , Permanyer , J , Irimia , M , Sebé-Pedrós , A & Solana , J 2021 , ' ACME dissociation : a versatile cell fixation-dissociation method for single-cell transcriptomics ' , Genome Biology , vol. 22 , no. 1 , 89 . https://doi.org/10.1186/s13059-021-02302-5en
dc.identifier.issn1474-7596
dc.identifier.urihttp://aura-test.abdn.ac.uk/handle/2164/19315
dc.descriptionFunding Information: We thank Helen Ferry and Liam Hardy at the Experimental Medicine Division Flow Cytometry Facility at the Nuffield Department of Clinical Medicine (University of Oxford), Michal Maj at the Flow Cytometry Facility at the Dunn School of Pathology (University of Oxford), and Òscar Fornas at the Centre for Genomic Regulation/Universitat Pompeu Fabra FACS Unit (Barcelona) for their help and advice with flow cytometry. We thank Zhong Yap from the Aboobaker Lab at Oxford University for the help with irradiation. We thank Alistair McGregor at Oxford Brookes University for providing useful comments and discussion about the dissociation; Teresa Adell of the Department of Genetics, University of Barcelona; Markus Grohme at MPI-CBG (Dresden); and Christopher Laumer at the European Bioinformatics Institute (Cambridge) for the discussion, advice on the protocol, and comments on the manuscript. We thank Mireya Plass at the Centre for Regenerative Medicine of Barcelona for the computational analysis advice and for the critical review of the manuscript. The review history is available as Additional file 6. Barbara Cheifet was the primary editor of this article and managed its editorial process and peer review in collaboration with the rest of the editorial team. Funding Information: This work was supported by an MRC grant (MR/S007849/1) and a Royal Society Grant (RGS\R1\191278) to JS. HG-C was supported by a Nigel Groome studentship from Oxford Brookes University. PA-C was supported by an EMBO Long Term Fellowship (ALTF-217-2018). JN was supported by funding from a BBSRC grant (BB/M011224/1) and the Osk. Huttunen Foundation (Doctoral grant). Publisher Copyright: © 2021, The Author(s).en
dc.format.extent4357318
dc.language.isoeng
dc.relation.ispartofGenome Biologyen
dc.subjectCombinatorial indexingen
dc.subjectDissociationen
dc.subjectFixationen
dc.subjectPlanarianen
dc.subjectRNA-seqen
dc.subjectSingle-cell transcriptomicsen
dc.subjectSPLiT-seqen
dc.subjectEcology, Evolution, Behavior and Systematicsen
dc.subjectGeneticsen
dc.subjectCell Biologyen
dc.subjectSupplementary Informationen
dc.titleACME dissociation : a versatile cell fixation-dissociation method for single-cell transcriptomicsen
dc.typeJournal articleen
dc.contributor.institutionUniversity of Aberdeen.Biological Sciencesen
dc.description.statusPeer revieweden
dc.identifier.doihttps://doi.org/10.1186/s13059-021-02302-5
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85103998605&partnerID=8YFLogxKen
dc.identifier.vol22en
dc.identifier.iss1en


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