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Green Bees : Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism

dc.contributor.authorGusachenko, Olesya N.
dc.contributor.authorWoodford, Luke
dc.contributor.authorBalbirnie-Cumming, Katharin
dc.contributor.authorCampbell, Ewan M.
dc.contributor.authorChristie, Craig R.
dc.contributor.authorBowman, Alan S.
dc.contributor.authorEvans, David J.
dc.date.accessioned2020-05-12T13:50:01Z
dc.date.available2020-05-12T13:50:01Z
dc.date.issued2020-05
dc.identifier162968695
dc.identifier3cceec8b-e7df-471f-a7a9-c80b86b19bea
dc.identifier32408550
dc.identifier85084786541
dc.identifier.citationGusachenko , O N , Woodford , L , Balbirnie-Cumming , K , Campbell , E M , Christie , C R , Bowman , A S & Evans , D J 2020 , ' Green Bees : Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism ' , Viruses , vol. 12 , no. 5 , 532 . https://doi.org/10.3390/v12050532en
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/2164/14309
dc.descriptionFunding: This work was supported by grant funding from Biotechnology and Biological Sciences Research Council (BBSRC): BBSRC BB/M00337X/2 and BB/I000828/1. C.R.C. was supported by a KTN BBSRC CASE studentship BB/M503526/1 (http://www.bbsrc.ac.uk). C.R.C was part-funded by the Scottish Beekeeping Association (https://www.scottishbeekeepers.org.uk/) and the Animal Health and Welfare program by the Scottish Government. E.M.C. was supported by the Veterinary Medicines Directorate, Department for Environment Food & Rural Affairs (Project # VM0517) (https://www.gov.uk/government/organisations/veterinary-medicines-directorate). Acknowledgments: We would like to express our gratitude to Robert J Paxton (Martin Luther University Halle-Wittenberg) for providing a reference sequence for the construction of DWV-B RG system, Marcus Bischoff and Gill McVee (University of St Andrews) for helping with microscopic imaging set up, Javier Tello (University of St Andrews) for help and advice on sample cryosectioning, Ivan Gusachenko (University of St Andrews) for obtaining a photo of EGFP-expressing pupa, Ashley Pearson (University of St Andrews) for assistance in molecular biology assays, and Kirsten Bentley (University of St Andrews) for the high practical and moral support throughout the project.en
dc.format.extent17
dc.format.extent4167694
dc.language.isoeng
dc.relation.ispartofVirusesen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subjectinsect virusesen
dc.subjecthoney beeen
dc.subjectpollinationen
dc.subjectvirus vectoren
dc.subjectVarroaen
dc.subjectRNA virusesen
dc.subjectDWVen
dc.subjectreverse geneticsen
dc.subjectReverse geneticsen
dc.subjectPollinationen
dc.subjectInsect virusesen
dc.subjectVirus vectoren
dc.subjectHoney beeen
dc.subjectQH301 Biologyen
dc.subjectInfectious Diseasesen
dc.subjectVirologyen
dc.subjectBiotechnology and Biological Sciences Research Council (BBSRC)en
dc.subjectBB/M00337X/2en
dc.subjectBB/I000828/1en
dc.subjectBB/M503526/1en
dc.subjectSupplementary Dataen
dc.subject.lccQH301en
dc.titleGreen Bees : Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropismen
dc.typeJournal articleen
dc.contributor.institutionUniversity of Aberdeen.Centre for Genome-Enabled Biology and Medicineen
dc.contributor.institutionUniversity of Aberdeen.Medical Sciencesen
dc.contributor.institutionUniversity of Aberdeen.Biological Sciencesen
dc.description.statusPeer revieweden
dc.identifier.doihttps://doi.org/10.3390/v12050532
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85084786541&partnerID=8YFLogxKen
dc.identifier.urlhttp://www.mdpi.com/1999-4915/12/5/532/s1en
dc.identifier.vol12en
dc.identifier.iss5en


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