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Structural basis of the filamin A actin-binding domain interaction with F-actin

dc.contributor.authorIwamoto, Daniel V.
dc.contributor.authorHuehn, Andrew
dc.contributor.authorSimon, Bertrand
dc.contributor.authorHuet-Calderwood, Clotilde
dc.contributor.authorBaldassarre, Massimiliano
dc.contributor.authorSindelar, Charles V.
dc.contributor.authorCalderwood, David A.
dc.date.accessioned2019-03-17T00:02:10Z
dc.date.available2019-03-17T00:02:10Z
dc.date.issued2018-10
dc.identifier131533954
dc.identifierd8db06ab-b359-4565-81b6-9f864001947d
dc.identifier30224736
dc.identifier85053504260
dc.identifier000446468200004
dc.identifier.citationIwamoto , D V , Huehn , A , Simon , B , Huet-Calderwood , C , Baldassarre , M , Sindelar , C V & Calderwood , D A 2018 , ' Structural basis of the filamin A actin-binding domain interaction with F-actin ' , Nature Structural & Molecular Biology , vol. 25 , pp. 918-927 . https://doi.org/10.1038/s41594-018-0128-3en
dc.identifier.issn1545-9985
dc.identifier.otherORCID: /0000-0002-2498-4767/work/76976090
dc.identifier.urihttp://hdl.handle.net/2164/12054
dc.descriptionCryo-EM reconstructions were deposited in the Electron Microscopy Data Bank with the following accession numbers: F20-F-actin-FLNaABD, EMD-7833; F20-F-actin-FLNaABD-Q170P, EMD-7832; F20-F-actin-FLNaABD-E254K, EMD-8918; Krios-F-actin-FLNaABD-E254K, EMD-7831. The corresponding FLNaABD-E254K filament model was deposited in the PDB with accession number 6D8C. Source data for F-actin-targeting analyses (Figs. 2c,d,g,h, 3b,c,e,f, 4d,e, 5c,d, and 6a,b) and co-sedimentation assays (Figs. 5g and 6d) are available with the paper online. Other data are available from the corresponding author upon reasonable request. We thank Z. Razinia for generating numerous FLNa constructs, S. Wu for expertise in using the Krios microscope, J. Lees for advice on model refinement, and M. Lemmon for helpful comments in preparing the manuscript. We also thank the Yale Center for Research Computing for guidance and use of the Farnam Cluster, as well as the staff at the YMS Center for Molecular Imaging for the use of the EM Core Facility. This work was funded by grants from the National Institutes of Health (R01-GM068600 (D.A.C.), R01-NS093704 (D.A.C.), R37-GM057247 (C.V.S.), R01-GM110530 (C.V.S.), T32-GM007324, T32-GM008283) and an award from American Heart Association (15PRE25700119 (D.V.I.)).en
dc.format.extent10
dc.format.extent2450296
dc.language.isoeng
dc.relation.ispartofNature Structural & Molecular Biologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subjectactinen
dc.subjectcryoelectron microscopeen
dc.subjectQH301 Biologyen
dc.subjectSupplementary Dataen
dc.subject.lccQH301en
dc.titleStructural basis of the filamin A actin-binding domain interaction with F-actinen
dc.typeJournal articleen
dc.contributor.institutionUniversity of Aberdeen.Medical Sciencesen
dc.description.statusPeer revieweden
dc.identifier.doi10.1038/s41594-018-0128-3
dc.date.embargoedUntil2019-03-17
dc.identifier.vol25en


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